Journal: bioRxiv

Article Title: Defining characteristics of mesenchymal stem cell-derived matrix-bound nanovesicles compared to conditioned culture medium extracellular vesicles

doi: 10.64898/2026.05.05.722048

Figure Lengend Snippet: (A) Representative images of Sirius Red-stained MSC sheets after 10 days of in vitro culture in L-ascorbic acid-2-phosphate. (B) Quantification of eluted Sirius Red dye from MSC sheets (n=6). Significance was calculated using a one-way ANOVA with a Tukey’s post-hoc test. (C) Representative SEM images of MSC sheets demonstrating vesicle-like structures on cells and ECM fibers. Potential MBV are indicated with white arrows.

Article Snippet: Two days later, normal growth medium was supplemented with 50 μM L-ascorbic acid-2-phosphate sesquimagnesium salt hydrate (Tokyo Chemical Industry, A2521) to induce cell-driven extracellular matrix deposition in vitro and cells were cultured for an additional 10 days.

Techniques: Staining, In Vitro